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Pharmacological alteration of Cntfr and Syt1 contacts At 14 days in vitro (DIV) neurons were incubated either in conditioned medium only (control), or in conditioned medium supplemented with 8 nM ciliary neurotrophic factor (CNTF, C-245 Alomone Labs) for 2 hours or 24 hours, or 3 M tetrodotoxin (TTX, 1069 Tocris) for 2 hours

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Ensure these SOPs are readily accessible and regularly reviewed

DOI:10.4103/1673-5374.320969

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If suspecting degradation, obtain fresh vial from reliable source comparing response