doi: 10.1016/S0021-9258(19)40184-1 37 FalkinhamJ
Successively the cells were stimulated overnight with TNF- (100 ng/mL), washed with Dulbecco's PBS added with 2% BSA (Bovine Serum Albumine, fraction V, Sigma-Aldrich) and CaCl 2 -MgCl 2 0,7 mM (Sigma-Aldrich), and then incubated with mouse mAb anti-human VCAM1 (Sigma-Aldrich) 5 g/mL for 90 min at RT
In experimental models it is used to explore mechanisms of tissue regeneration, angiogenesis, inflammatory modulation and maintenance of gastrointestinal barrier integrity
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