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Briefly, approximately 1.510 5 host cells (A549, RAW 264.7, PBL-985, and PBL-985 nox2 -KO cell lines) were seeded per well in 12-well plates and cultured in DMEM + 5% FBS at 37C and 5% CO2 for 24 h until reaching 95% confluence, equivalent to approximately 3 10 5 cells

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It also induces the expression of suppressor of cytokine signaling-3, which is a negative regulator of leptin signaling [13]

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doi: 10.1053/j.gastro.2021.12.288