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Aliquots were spotted onto P81 phosphocellulose filters, which were then washed three times for 10 min each in 75 mM phosphoric acid, followed by a 5-min wash in methanol
Other groups have considered specific cell processes, such as anoikis, a form of apoptosis that has been implicated in cancer tumor progression and metastasis and that remains poorly understood in CaP
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Int Immunopharmacol 67:119128 Maes M, Lambrechts J, Bosmans E, Jacobs J, Suy E, Vandervorst C, De Jonckheere C, Minner B, Raus J (1992) Evidence for a systemic immune activation during depression: results of leukocyte enumeration by flow cytometry in conjunction with monoclonal antibody staining
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