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The most widely used cysteine detection method, Ellman's reagent (DTNB), reacts with virtually any free thiol in the sampleglutathione, coenzyme A, reduced protein sulfhydryls, even the cysteine residues exposed on the surface of albuminand reports their combined concentration as "cysteine." In a typical mammalian cell lysate, glutathione is present at millimolar concentrations, roughly 100- to 1,000-fold higher than free cysteine