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Trypsin (v5280, Promega, Madison, WI, United States) was then used to digest proteins into peptides (1:50 ratio of trypsin to protein (wt/wt)) and the tryptic digests were suspended in water/acetonitrile/formic acid (95:3:2) solution and analyzed in mass spectrometry

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By integrating the network graph and the topological attributes table, we identified five key targets associated with UC, including REN, LNPEP, HLA-A, OXTR, and SIRT1