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Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)

Eur J Clin Pharmacol 8:5562 Fulton B, James O, Rawlins M (1979) The influence of age on the pharmacokinetics of paracetamol [proceedings]

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There is also evidence showing a significant decrease in TAS in one study [55], while other research indicates lower levels, but without reaching statistical significance [68,69]