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Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

Concurrent CYP3A4 Inhibitor Use Patients already taking strong CYP3A4 inhibitors (ketoconazole, itraconazole, ritonavir, clarithromycin) have elevated dutasteride levels

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482 Astrocytic RASopathy Mutations of rat sarcoma virus or RAS proteins are behind a class of disorders known as RASopathies