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As expected for the intended effect of ADT, metabolite set enrichment analysis (MSEA) of the significantly different metabolites revealed steroid hormone biosynthesis pathways, including androstenedione metabolism as well as androgen and estrogen metabolism, to be overrepresented by metabolites that were significantly different between +ADT and ADT groups (Figure 1E)

4 Phytochemical compounds in garlic Approximately 184 diverse types of active secondary metabolites are found in garlic ( 4.1 Bioactive compounds of garlic Due to its many phytochemicals, minerals, and dietary fiber, garlic is considered a valuable spice

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Quantitative real-time RT-PCR was performed as described, and primers sequences were written in the report 7