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Increased expression of III tubulin, the protein related to axonal maturation, was observed in a rat corneal injury model 17

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Procedure: Clean scalp thoroughly Apply numbing cream if desired (optional for 0.5-1.0mm) Microneedle in multiple directions (vertical, horizontal, diagonal) Apply peptide solution immediately after Gentle pressure/massage to work peptides into channels Leave on scalp (don't rinse) What to avoid: Don't microneedle over active infections or inflamed areas Don't share microneedling devices Don't use dull needles (replace dermaroller every 3-4 uses) Don't apply harsh products (alcohol, fragrances) immediately after Best practices: Use a dermapen if possible (more consistent depth, less painful than dermaroller) Sanitize device before and after each use Take progress photos before starting protocol Combining peptides with other hair loss treatments Peptides work synergistically with established hair loss treatments

The Vasireddi et al

Users adjust the amount based on the severity of the injury and total body weight