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The expression levels of cross-linking enzymes and structural proteins produced by cellssuch as keratin laminin, filaggrin (FLG), transglutaminase-1, and keratin filamentthat are related to skin barrier function are downregulated under the action of ROS, thereby impairing skin barrier function ( FIGURE 5 4 Therapeutic strategies based on ROS regulation With the deepening understanding of the role of ROS in skin diseases, various therapeutic strategies targeting ROS regulation have been developed