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Briefly, the ELISA plate (Corning 3690) was coated with murine anti-AMG 479, clone 3 at 2 g/mL in 1 PBS and incubated overnight at 4 C

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The formation of ROS is also a consequence of defective mitochondria that, additionally, leads to the activation of the inflammasome and of the pro-inflammatory nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B)

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