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Tissues were incubated in a 15 ml Falcon tube with 1 ml of collagenase P solution for 15 min at 37 C, followed by addition of 12 ml of the cold G-solution (Sigma Aldrich) and centrifugation at 1,620 rpm at room temperature

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This study specifically aimed to minimize bias through rigorous methodology

Thomas MK, Nikooienejad A, Bray R et al (2021) Dual GIP and GLP-1 receptor agonist tirzepatide improves beta-cell function and insulin sensitivity in type 2 diabetes

World Health Organization (WHO)

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