The labeled cells were washed and harvested in TNM buffer (10 mM Tris pH 7.5, 10 mM NaCl, 1.5 mM MgCl 2 , 0.1% Tween)
Richter et al., 2017b)
10.1002/ana.25356 77 KennyE
The influence of macro and trace elements on sperm quality
The building blocks within the software were used to identify and calculate the mean dye parameters per well: number of nuclei, MitoSOX Red, or PI mean intensity/cell
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