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TJs play an important role in preventing the downward diffusion of apical surface receptors to the basolateral membrane thereby maintaining cellular polarity and ensuring IECs can detect external stimuli in the gut lumen and stimulate the appropriate downstream response (Knig et al

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Western blot Total proteins from ovarian tissues and cells were extracted using radioimmunoprecipitation assay lysis buffer (Beyotime) and quantified by bicinchoninic acid method