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Cysteine may be obtained from extracellular cysteine or cystine and, in some tissues, from the methionine cycle through the transsulfuration pathway

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calculated as the sum of reduced GSH and two times the amount of oxidized glutathione (GSH + 2 GSSG)) levels in the serum and in the tumour interstitial fluid (TIF), the extracellular compartment surrounding tumours