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In cognitive peptides: substitute when possible in vivo tests by cerebral organoids or primary neuronal cultures ( Replacement ), optimise experimental plan to minimise the number of animals ( Reduction ), use appropriate anaesthesia and analgesia during invasive procedures ( Refinement )

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The acupoint Shenmen (HT7) is frequently incorporated into therapeutic protocols and is commonly combined with other acupoints, such as Yongquan (KI1) and Taixi (KI3), to address specific symptom profiles [73, 74]

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A gut-brain neural circuit for nutrient sensory transduction

2017;(4)doi:10.1002/14651858.cd012010.pub2 Burkart J, Haigler S, Caruana R, Hylander B