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Nutrition approach: Focus on protein at every meal (aim for 25-30g per meal minimum) Reduce ultra-processed food intake by 50% or more Eat vegetables with every meal to support gut health Stay hydrated with 64+ ounces of water daily Sleep optimization: Target 7-9 hours nightly Consistent sleep and wake times No screens 60 minutes before bed Cool, dark sleeping environment Movement: Daily walking, 20-30 minutes minimum Light resistance training 2-3 times per week No intense exercise that triggers compensatory hunger Tracking: Daily food journal noting hunger levels (1-10 scale) before and after meals Weekly weight check (same time, same conditions) Side effect log for provider discussions Protocol 2: weeks 5-12 (appetite optimization) Goal: Leverage the first therapeutic dose for maximum appetite control

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Genome Biol 20:257 Sabater C, Ruiz L, Margolles A (2021) A machine learning approach to study glycosidase activities from Bifidobacterium

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doi: 10.2174/13816128113199990559 61

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