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RNA extraction and RT-PCR for gene expression analysis To determine the effect of HT stress on the transcription levels of Calvin cycle enzyme genes and antioxidant enzyme genes and their changes after GSH application, we tested photosynthetic genes including those encoding ribulose-1,5-bisphosphate carboxylase/oxygenase activase ( RCA ), RBCL and RBCS involved in CO 2 fixation

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Ashley, director of the Office of Compliance in the FDA's Center for Drug Evaluation and Research, said in a press release