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Science, 350(6261), 674-677

A standard volume of master mix was added to each reaction tube, and Ub was added to initiate the ubiquitination reaction containing 100M Ub, 1M SKP1-FBXO22, 0.5M *NSD2 PWWP1, 0.5M compound, 0.1M UBA1, 1M UBE2R2, 1M UBE2D2 or 1M UBE2L3, and 1M ARIH1, 1M NEDD8~CUL1-RBX1, and 5mM MgATP in reaction buffer