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*Correspondence: Giulia Guerriero, Department of Biology, Comparative Endocrinology Lab, Universit degli Studi di Napoli Federico II, Via Mezzocannone, 8, Naples 80134, Italy e-mail: [email protected] This article was submitted to Experimental Endocrinology, a section of the journal Frontiers in Endocrinology

ensure all reagents and equipment are at the appropriate temperature before starting the assay

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