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Topaz trained-based autopicking in cryoSPARC (v4.5.0) was implemented to select particle images, resulting in 3,216,504, 3,182,579, and 6,617,058 particles respectively from each dataset 61,63

The trigger is usually reaching a dose that exceeds what their current concentration can deliver in a single syringe draw

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NYHA class IV was exclusion criteria in ELIXA, LEADER and SUSTAIN-6 trials