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Following digestion, peptides were desalted using Oasis HLB (Elution format, Waters)

Chronic metabolic conditions such as obesity, diabetes, cardiometabolic, and fatty liver disease are frequently associated with alterations in the gut metabolome 8

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however, consistent fasting practices may lead to hormonal adaptations that support more efficient energy balance and metabolic health [53]

It was demonstrated that Lactobacillus reuteri supplementation improves thyroid function in mice by increasing free T4 and thyroid mass (241)