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Strengths of the study included longer follow-up to assess disease progression and spectral higher resolution (at 7T)

Both endothelial cells and aortic sections were treated with 0.5% (v/v) Triton X-100 for 15 min, followed by three washes with PBS, each time for 5 min, and then blocked with 5% (v/v) bovine serum albumin (BSA, Sigma-Aldrich, B2064) at room temperature for 1 h

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27 Homemade diets are increasing in popularity and are a valid diet option when properly formulated to meet the dogs individual nutrient requirements

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