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Since decreased Parasutterella relative abundance may accompany increased stress 80,81 , the increased Parasutterella relative abundance that occurred in this study following diet switch likely arose from the weight loss-mediated negative energy state

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The methylenetetrahydrofolate reductase enzyme(MTHFR), which is encoded by the MTHFR gene, is a critical enzyme that regulates the metabolism of folate and methionine, both of which are important factors in DNA methylation

Additionally, Biesinger et al

Repeated freezing and thawing damages peptides through ice crystal formation