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The checkpoint inhibitor agents included in the study were CTLA-4 inhibitors, PD-1 inhibitors, PD-L1 inhibitors, and combination treatment with a CTLA-4 inhibitor (ipilimumab) and a PD-1 inhibitor (nivolumab)

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In addition, SIRT1, an NAD + -consumed enzyme, counteracts the effect of the circadian complex by deacetylating BMAL1 and the histones in the promoter region of other regulated genes

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These analytical methods provide researchers with confidence in the quality and consistency of their experimental materials