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To facilitate Gibson assembly into the pET-28a(+) vector, short sequences were added at the 5 end ( CTCTAGAAATAATTTTGTTTAACTTTAA-GAAGGAGATATACC ) and 3 end (GATCCGGCTGCTAACAAAGCCCGAAAG) of each gene

LCMV is a non-cytopathic virus that replicates slowly, which favors its persistence due to the failure of the host to mount an efficient cytotoxic T cell response 89,94

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ROS are involved in the regulation of a wide range of physiological and pathological processes 15,16

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