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excessive melanin leads to hyperpigmentation, dark spots, and uneven skin tone

The hypoglycemic and antioxidant effects of polysaccharides from the petioles and pedicels of Euryale ferox Salisb

PMID 21631324

Yang L, Liu Y, Sun L, Zhao C, Chen G, Zhao Y

Ultra-filtration (to wash out un-reacted free trisulfides) Trisulfide-pretreated HGF solutions were loaded into Amicon ultra-filtration devices (with a 10-kDa MWCO polyethersulfone (PES)-membrane) and spun at the recommended centrifugal force (typically 6000-7000 rpm) for 1030 min at 4 or until the desired volume was reached