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Membranes were blocked for 1 hr in blocking buffer (5% w/v solution of non-fat dry milk in TBS-T: 1X Tris-buffered saline (20 mM Tris, 150 mM NaCl) supplemented with 0.1% Tween-20 detergent)

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Zero adverse events across all groups No adverse events were reported in any of the three groups over the full 6-month treatment period

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Ishaq HM, Mohammad IS, Guo H, Shahzad M, Hou YJ, Ma C et al (2017) Molecular estimation of alteration in intestinal microbial composition in Hashimotos thyroiditis patients

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Finally, the Geographically Weighted Regression (GWR) model is used to cluster the parks based on their cooling performance

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This process produces ATP efficiently while consuming oxygen, creating a hypoxic environment at the gut surface that favors beneficial anaerobic bacteria while inhibiting potentially harmful aerobic pathogens

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