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Hypoglycaemia endpoints were analysed using a negative binomial model with log-link function, including treatment, region and the logarithm of the time period in which a participant was exposed to the trial product as offset

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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

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