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The assay was designed to demonstrate the biochemical activity of c-met binding sites including Y198 and Y250 (in K1 and K2 domains of HGF, respectively) by significant changes in optical absorbance (expressed as relative unit) within a range from a baseline level (a negative control without HGF

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All samples were diluted in the SPR buffer to a volume of 200 l and injected using the OneStep gradient injection system at a flow rate of 150 l/min

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Studies that show no link between a genetic variant and a trait are also important to consider

Its used topically in some protocols but more commonly injected for systemic effect