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Transmission electron microscopy HCT116 cells were treated with 5 M 5FU for 72 h before being pelleted and fixed with 3% glutaraldehyde (Agar Scientific) in cacodylate (CACO)

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Similar to the hippocampus, a significant portion of cerebellar Purkinje cells in the CN group exhibited dark appearance with a significant cell shrinkage and cytoplasmatic condensation, which is indicative of cellular stress or degeneration

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