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A fluorescence activated cell sorter (FACS) analysis was performed using annexin V, which specifically and strongly binds to cell surface PI in order to confirm whether apoptotic or necrotic cell death was observed [30]

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PDX3 tumors treated with COJEC that relapsed or regrew with acquired resistance 12 had significantly higher levels of the antioxidant pathways compared to PDX3 tumors that were cured (Fig