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lactis strains transformed with the different plasmids used in this work were routinely grown at 30 C in M-17 liquid medium (Millipore - Merck KGaA, Darmstadt, Germany) supplemented with 0.5% glucose (Sigma-Aldrich - Merck KGaA, Darmstadt, Germany) (GM-17 medium) without agitation, or in plates containing the same medium solidified with 1.5% agar (Carlo Erba, Cornaredo (MI), Italy)

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Of those who experience migraine, 2% experience chronic migraine