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The supernatant was incubated with 100 L anti-Flag M2 affinity agarose gel (Sigma, A2220) at 4 C for 2.5 h

For improved production of vanillin- - glucoside, an essential gene was placed under the control of a synthetic promoter, inducible in the presence of the vanillin- - glucoside precursor [21]

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One of the earliest tissues affected by the onset of the soft tissue calcification with vitamin D toxicity is the kidney

GPx7 likes to interact with the domain of PDI, and intramolecular cooperation between the two redox-active sites of PDI increases the activity of the Ero1/GPx7/PDI triplet, thereby promoting protein folding mechanisms (Wang et al., 2014) (Figure 6)