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2.5.3 RT-qPCR calculations and data processing Real-time PCR Analysis Software (version 4.5.2, Fluidigm) was used to validate the PCR cycles with the default analysis settings for qPCR and melting curve analysis

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Butyric acid inhibits of m6A methyltransferase METTL3 expression in KGN cells, resulting in a decrease in FOSL2m6A methylation level and mRNA expression (185)

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