LiDPanJZhangYLiYJinSZhongCet al
Free Radic Biol Med 87:226236
Chromatographic conditions The chromatographic separation of N-Hcy-Lys and low-molecular-mass thiols in human plasma was obtained in 9 min
In-Vivo Models in the Literature Animal model work involving BAC Water spans rodent, large-animal, and (in some cases) primate studies
One looked at 16 patients with knee pain
For each pulldown or binding assay, 1 mg of lysate or 1 g of recombinant YBX1HA protein was diluted in the reaction buffer containing protease inhibitor (APExBIO, 1007), RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) and 260 l of 2 TENT buffer (20 mM Tris-HCl pH 8.0, 2 mM EDTA, 500 mM NaCl and 1% v/v Triton X-100) and polysome extraction buffer (20 mM Tris-HCl pH 7.5, 100 mM KCl, 5 mM MgCl 2 and 0.5% NP-40) in a final volume of 520 l