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The amounts of albumin inside the lysed cells, or recycled albumin in the medium, were quantified by ELISA, in which albumin was captured on an anti-human albumin antibody and detected with an ALP-conjugated anti-human albumin antibody

RNA Extraction and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted from cellular and cerebral samples using TRIzol reagent (Invitrogen, Carlsbad, CA, United States)

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