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Figure 2 Signaling pathways activated by TGR5 Activation of GS/cAMP pathway In membranes isolated from STC-1 cells, OA (10 M) caused a significant increase in the binding of [ 35 S]GTPS to G s as determined by the binding of [ 35 S]GTPS.G complexes to the corresponding G s antibody (10 2-fold increase in the binding) (Figure 3A)