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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader

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The advent of potent anti-inflammatory agents with their strong effects on reducing annualized relapsed rates has advanced the concept of progression independent of relapse activity (PIRA) (Lublin et al., 2022)

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It indicates growing public interest and awareness over time

This dysbiosis disrupts immune homeostasis by elevating proinflammatory cytokines and compromising immunosurveillance, thereby promoting ectopic lesion survival and growth