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In general, the longer the asparagus was blanched, the more nutrients it lost, though cooking it for too short a time resulted in hard stalks

After 24 h, cells were expanded into six-well dishes and selected in 1 g ml 1 puromycin for 3 days, then further expanded in nonselective medium

The first group function in glutathione homeostasis including amino acid uptake transporters (SLC7A11 and SLC6A9), GSH synthesizing enzymes (-GCLc and -GCLm), GSH metabolizing enzymes that eliminate ROS through GSSG/GSH oxidation-reduction cycle (GPx and GRx) and GSH complementary enzymes such as glutathione transferases (GST)