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(2) The standard curve To set up the assays standard curve, we diluted equivalent concentrations of peroxide standard with 0.1 M phosphate buffer at pH 7.0 to generate a range of standard concentrations from 0.1 to 2 mM H 2 O 2

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Jones et al., 2018b)

Because IGF-1 LR3 is a folded protein of roughly 9.1 kDa carrying three disulfide bridges rather than a short synthetic peptide, agitation is the main practical hazard: shear and air-liquid interface exposure drive aggregation, so swirl only and never shake

While some may notice improvements in hair thickness and vitality, copper peptides are not a guaranteed solution for hair loss