Since the neuronal correlates of trial-by-trial variation of self-timing emerged earlier in the cerebellum than in the striatum, the stochastic variation of self-timing might be primarily responsible for neuronal signals in the cerebellum [341]
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Potential concerns include B-vitamin accumulation (especially if dietary intake is adequate), transient injection-site reactions, and rare hypersensitivity to amino acid components
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Then, the media were discarded, and the cells were washed by PBS then the metabolites extracted using methanol: water (9:1, v/v) mixtures