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PMID 17227934

DOI References 1

Peptide-specific mixing considerations While the basic reconstitution process is universal, certain peptides have quirks that require special attention

And bacterial contamination becomes a risk once preservatives in the solution begin to degrade, which happens faster at higher temperatures

Draw the planned BAC water volume into a fresh syringe

For the aortic samples from aged mice, first, the aortas from different treatment groups were excised and other tissues were removed, leaving only the aortic endothelium