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Nuclei were stained with DAPI Fluoromount-G (SouthernBiotech)

First of all, we implemented a rigorous and comprehensive search strategy following Cochrane Guidelines for Systematic Reviews of Interventions ( CRD42022323788 )

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Patients reported that side-effects persisted during the semaglutide dose escalation, after which the symptoms subsided in all, but were still present at the time of discontinuation of semaglutide (timepoint 2)