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Pulldown assay and western blots EBV-transformed B cell clones were harvested and lysed in 1 lysis buffer (20 mM Tris-HCl pH 7.5, 1 mM EDTA, 150 mM NaCl, and 1% Triton X-100) supplemented with protease inhibitors (1 mM Na 3 VO4, 1 mM PMSF, and 2 g/mL Leupeptin)

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Stacking them requires specific caution because GLP-1 drugs alter gastric motility, insulin signaling, and appetite regulation