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Protein expression/Purification The vector, pJB-GST-HTS-HS-GFP was transformed into BL21 expression cells for expression and purification of GST-GFP protein

[9] According to the authors of the study, this effect can be explained by ergothioneine's ability to not only prevent, but actually repair damage done to DNA by UVA: "The increase in DNA synthesis in UV-irradiated EGT-treated cells at 48 h compared to irradiated controls, accompanied bythe increase in viability and reduction of apoptosis, suggests that EGT not only may prevent damage but also may supplement repair

Significant differences among different groups were determined by two-tailed Students t -test for two-group comparisons

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Methods: UC was induced in BALB/c mice with 3% DSS for 14 days

In biochemical and industrial contexts, it is commonly referred to simply as glutathione or by the abbreviation GSH when indicating its reduced form