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Negative control staining without the primary antibody was performed to confirm the antibody specificity

One unit of POD is the amount of enzyme required to catalyse the reduction of 1 M of H 2 O 2 , using guaiacol as the hydrogen donor, per minute under certain conditions, and was calculated using the enzyme coefficient 26.6 mM 1 cm 1

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However, these two natural GLP-1R agonists are rapidly digested by the enzyme DPP-4 and cleared by the kidneys within 1-2 minutes, making them unsuitable for direct therapeutic use

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