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Metabolites were separated on ACQUITY UPLC BEH 300 C18 (1,7 m, Waters) with a flow rate of 0.3 mL/min with gradient of running solvent A (0.1% formic acid in water) and running solvent B (0.1% formic acid in methanol): start with 100% B and holding over 5 minutes, minutes 5 to seven 99% B and ending from 7 to 10 minutes with 1% B