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To remove the cleaved tag and TEV protease, the dialysed protein was loaded onto an equilibrated 5-mL HisTrap HP column, and the flowthrough was collected
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Such bidirectional interaction between hyperandrogenism and insulin resistance/hyperinsulinemia likely plays a key role in the pathogenesis of PCOS 12,13,14 , since elevated testosterone promotes obesity and insulin resistance/hyperinsulinemia by altering body composition and insulin signaling in skeletal muscle and adipose tissue, while hyperinsulinemia contributes to increase androgen levels via ovarian-dependent mechanisms 5
